expression construct rrm2-gfp (OriGene)
Structured Review

Expression Construct Rrm2 Gfp, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rrm2+gfp/rrm2++nm+001165931++human+cdna+clone+sc326997/pmc05650473-9-0-6
Average 90 stars, based on 1 article reviews
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1) Product Images from "Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS"
Article Title: Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS
Journal: eLife
doi: 10.7554/eLife.27574
Figure Legend Snippet: ( A ) Line graphs showing mean abundance profiles for Cyclin A2 (CycA), Cyclin B1, Cyclin B2, and GAPDH. Grey ribbons indicate 1 standard deviation from the mean. ( B ) Mean abundance profile for RRM2. ( C ) Flow cytometry analysis RRM2 levels vs. DNA content. ( D ) Flow cytometry-based comparison of beta-tubulin (negative control, left) and RRM2 (right) levels in CycA+ (red) vs. CycA- (blue) prometaphase cells. ( E ) Violin plots showing CycA, histone H3, and RRM2 levels in cells treated with either DMSO or microtubule drugs that activate the spindle assembly checkpoint (nocodazole, monastrol, taxol). ( F ) Violin plots showing levels of RRM2 in cells treated with either vehicle control (DMSO), MG132, apcin + proTAME, or MLN4924.
Techniques Used: Standard Deviation, Flow Cytometry, Comparison, Negative Control, Control
Figure Legend Snippet:
Techniques Used: Expressing, Construct
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Over Expression:Article Title: Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS Article Snippet: .. For overexpression experiments, HeLa cells were transfected with either GFP-fibrillarin, or Article Title: Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS Article Snippet: .. For overexpression experiments, HeLa cells were transfected with either GFP-fibrillarin, or Transfection:Article Title: Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS Article Snippet: .. For overexpression experiments, HeLa cells were transfected with either GFP-fibrillarin, or Article Title: Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS Article Snippet: .. For overexpression experiments, HeLa cells were transfected with either GFP-fibrillarin, or Expressing:Article Title: Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS Article Snippet: Antibody , mouse anti-tubulin (DM1a) , Sigma , RRID: AB_477593 , . .. DNA, Expression construct , Construct:Article Title: Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS Article Snippet: Antibody , mouse anti-tubulin (DM1a) , Sigma , RRID: AB_477593 , . .. DNA, Expression construct , |