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expression construct rrm2-gfp  (OriGene)


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    Structured Review

    OriGene expression construct rrm2-gfp
    ( A ) Line graphs showing mean abundance profiles for Cyclin A2 (CycA), Cyclin B1, Cyclin B2, and GAPDH. Grey ribbons indicate 1 standard deviation from the mean. ( B ) Mean abundance profile <t>for</t> <t>RRM2.</t> ( C ) Flow cytometry analysis RRM2 levels vs. <t>DNA</t> content. ( D ) Flow cytometry-based comparison of beta-tubulin (negative control, left) and RRM2 (right) levels in CycA+ (red) vs. CycA- (blue) prometaphase cells. ( E ) Violin plots showing CycA, histone H3, and RRM2 levels in cells treated with either DMSO or microtubule drugs that activate the spindle assembly checkpoint (nocodazole, monastrol, taxol). ( F ) Violin plots showing levels of RRM2 in cells treated with either vehicle control (DMSO), MG132, apcin + proTAME, or MLN4924.
    Expression Construct Rrm2 Gfp, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rrm2+gfp/rrm2++nm+001165931++human+cdna+clone+sc326997/pmc05650473-9-0-6
    Average 90 stars, based on 1 article reviews
    expression construct rrm2-gfp - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS"

    Article Title: Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS

    Journal: eLife

    doi: 10.7554/eLife.27574

    ( A ) Line graphs showing mean abundance profiles for Cyclin A2 (CycA), Cyclin B1, Cyclin B2, and GAPDH. Grey ribbons indicate 1 standard deviation from the mean. ( B ) Mean abundance profile for RRM2. ( C ) Flow cytometry analysis RRM2 levels vs. DNA content. ( D ) Flow cytometry-based comparison of beta-tubulin (negative control, left) and RRM2 (right) levels in CycA+ (red) vs. CycA- (blue) prometaphase cells. ( E ) Violin plots showing CycA, histone H3, and RRM2 levels in cells treated with either DMSO or microtubule drugs that activate the spindle assembly checkpoint (nocodazole, monastrol, taxol). ( F ) Violin plots showing levels of RRM2 in cells treated with either vehicle control (DMSO), MG132, apcin + proTAME, or MLN4924.
    Figure Legend Snippet: ( A ) Line graphs showing mean abundance profiles for Cyclin A2 (CycA), Cyclin B1, Cyclin B2, and GAPDH. Grey ribbons indicate 1 standard deviation from the mean. ( B ) Mean abundance profile for RRM2. ( C ) Flow cytometry analysis RRM2 levels vs. DNA content. ( D ) Flow cytometry-based comparison of beta-tubulin (negative control, left) and RRM2 (right) levels in CycA+ (red) vs. CycA- (blue) prometaphase cells. ( E ) Violin plots showing CycA, histone H3, and RRM2 levels in cells treated with either DMSO or microtubule drugs that activate the spindle assembly checkpoint (nocodazole, monastrol, taxol). ( F ) Violin plots showing levels of RRM2 in cells treated with either vehicle control (DMSO), MG132, apcin + proTAME, or MLN4924.

    Techniques Used: Standard Deviation, Flow Cytometry, Comparison, Negative Control, Control


    Figure Legend Snippet:

    Techniques Used: Expressing, Construct

    Related Articles

    Over Expression:

    Article Title: Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS
    Article Snippet: .. For overexpression experiments, HeLa cells were transfected with either GFP-fibrillarin, or RRM2-GFP (Origene RG205718) using Lipofectamine 3000 (Life Technologies). .. Transfected cells were incubated for 24 hr before harvesting for immunostaining using mouse anti-GFP (Roche, RRID: AB_390913) and rabbit anti-RRM2 primary antibodies (HPA056994, RRID: AB_2683304).

    Article Title: Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS
    Article Snippet: .. For overexpression experiments, HeLa cells were transfected with either GFP-fibrillarin, or RRM2-GFP (Origene RG205718) using Lipofectamine 3000 (Life Technologies). .. Transfected cells were incubated for 24 hr before harvesting for immunostaining using mouse anti-GFP (Roche, RRID: AB_390913 ) and rabbit anti-RRM2 primary antibodies (HPA056994, RRID: AB_2683304 ).

    Transfection:

    Article Title: Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS
    Article Snippet: .. For overexpression experiments, HeLa cells were transfected with either GFP-fibrillarin, or RRM2-GFP (Origene RG205718) using Lipofectamine 3000 (Life Technologies). .. Transfected cells were incubated for 24 hr before harvesting for immunostaining using mouse anti-GFP (Roche, RRID: AB_390913) and rabbit anti-RRM2 primary antibodies (HPA056994, RRID: AB_2683304).

    Article Title: Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS
    Article Snippet: .. For overexpression experiments, HeLa cells were transfected with either GFP-fibrillarin, or RRM2-GFP (Origene RG205718) using Lipofectamine 3000 (Life Technologies). .. Transfected cells were incubated for 24 hr before harvesting for immunostaining using mouse anti-GFP (Roche, RRID: AB_390913 ) and rabbit anti-RRM2 primary antibodies (HPA056994, RRID: AB_2683304 ).

    Expressing:

    Article Title: Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS
    Article Snippet: Antibody , mouse anti-tubulin (DM1a) , Sigma , RRID: AB_477593 , . .. DNA, Expression construct , RRM2-GFP , Origene , RG205718 , . ..

    Construct:

    Article Title: Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS
    Article Snippet: Antibody , mouse anti-tubulin (DM1a) , Sigma , RRID: AB_477593 , . .. DNA, Expression construct , RRM2-GFP , Origene , RG205718 , . ..



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    OriGene expression construct rrm2-gfp
    ( A ) Line graphs showing mean abundance profiles for Cyclin A2 (CycA), Cyclin B1, Cyclin B2, and GAPDH. Grey ribbons indicate 1 standard deviation from the mean. ( B ) Mean abundance profile <t>for</t> <t>RRM2.</t> ( C ) Flow cytometry analysis RRM2 levels vs. <t>DNA</t> content. ( D ) Flow cytometry-based comparison of beta-tubulin (negative control, left) and RRM2 (right) levels in CycA+ (red) vs. CycA- (blue) prometaphase cells. ( E ) Violin plots showing CycA, histone H3, and RRM2 levels in cells treated with either DMSO or microtubule drugs that activate the spindle assembly checkpoint (nocodazole, monastrol, taxol). ( F ) Violin plots showing levels of RRM2 in cells treated with either vehicle control (DMSO), MG132, apcin + proTAME, or MLN4924.
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    OriGene rrm2 gfp
    ( A ) Line graphs showing mean abundance profiles for Cyclin A2 (CycA), Cyclin B1, Cyclin B2, and GAPDH. Grey ribbons indicate 1 standard deviation from the mean. ( B ) Mean abundance profile <t>for</t> <t>RRM2.</t> ( C ) Flow cytometry analysis RRM2 levels vs. <t>DNA</t> content. ( D ) Flow cytometry-based comparison of beta-tubulin (negative control, left) and RRM2 (right) levels in CycA+ (red) vs. CycA- (blue) prometaphase cells. ( E ) Violin plots showing CycA, histone H3, and RRM2 levels in cells treated with either DMSO or microtubule drugs that activate the spindle assembly checkpoint (nocodazole, monastrol, taxol). ( F ) Violin plots showing levels of RRM2 in cells treated with either vehicle control (DMSO), MG132, apcin + proTAME, or MLN4924.
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    OriGene dna, expression construct rrm2-gfp
    ( A ) Line graphs showing mean abundance profiles for Cyclin A2 (CycA), Cyclin B1, Cyclin B2, and GAPDH. Grey ribbons indicate 1 standard deviation from the mean. ( B ) Mean abundance profile <t>for</t> <t>RRM2.</t> ( C ) Flow cytometry analysis RRM2 levels vs. <t>DNA</t> content. ( D ) Flow cytometry-based comparison of beta-tubulin (negative control, left) and RRM2 (right) levels in CycA+ (red) vs. CycA- (blue) prometaphase cells. ( E ) Violin plots showing CycA, histone H3, and RRM2 levels in cells treated with either DMSO or microtubule drugs that activate the spindle assembly checkpoint (nocodazole, monastrol, taxol). ( F ) Violin plots showing levels of RRM2 in cells treated with either vehicle control (DMSO), MG132, apcin + proTAME, or MLN4924.
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    Image Search Results


    ( A ) Line graphs showing mean abundance profiles for Cyclin A2 (CycA), Cyclin B1, Cyclin B2, and GAPDH. Grey ribbons indicate 1 standard deviation from the mean. ( B ) Mean abundance profile for RRM2. ( C ) Flow cytometry analysis RRM2 levels vs. DNA content. ( D ) Flow cytometry-based comparison of beta-tubulin (negative control, left) and RRM2 (right) levels in CycA+ (red) vs. CycA- (blue) prometaphase cells. ( E ) Violin plots showing CycA, histone H3, and RRM2 levels in cells treated with either DMSO or microtubule drugs that activate the spindle assembly checkpoint (nocodazole, monastrol, taxol). ( F ) Violin plots showing levels of RRM2 in cells treated with either vehicle control (DMSO), MG132, apcin + proTAME, or MLN4924.

    Journal: eLife

    Article Title: Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS

    doi: 10.7554/eLife.27574

    Figure Lengend Snippet: ( A ) Line graphs showing mean abundance profiles for Cyclin A2 (CycA), Cyclin B1, Cyclin B2, and GAPDH. Grey ribbons indicate 1 standard deviation from the mean. ( B ) Mean abundance profile for RRM2. ( C ) Flow cytometry analysis RRM2 levels vs. DNA content. ( D ) Flow cytometry-based comparison of beta-tubulin (negative control, left) and RRM2 (right) levels in CycA+ (red) vs. CycA- (blue) prometaphase cells. ( E ) Violin plots showing CycA, histone H3, and RRM2 levels in cells treated with either DMSO or microtubule drugs that activate the spindle assembly checkpoint (nocodazole, monastrol, taxol). ( F ) Violin plots showing levels of RRM2 in cells treated with either vehicle control (DMSO), MG132, apcin + proTAME, or MLN4924.

    Article Snippet: DNA, Expression construct , RRM2-GFP , Origene , RG205718 , .

    Techniques: Standard Deviation, Flow Cytometry, Comparison, Negative Control, Control

    Journal: eLife

    Article Title: Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS

    doi: 10.7554/eLife.27574

    Figure Lengend Snippet:

    Article Snippet: DNA, Expression construct , RRM2-GFP , Origene , RG205718 , .

    Techniques: Expressing, Construct